Knockdown of CXCL3-inhibited apoptosis and inflammation in lipopolysaccharide-treated BEAS-2B and HPAEC through inactivating MAPKs pathway

Background: CXCL3 (C-X-C motif chemokine ligand 3) is a member of chemokines family, which binds to the receptor to recruit neutrophils to lungs, thus participating in the pathogenesis of asthmatic lung. The role of CXCL3 in sepsis-induced acute lung injury is investigated here.Methods: Human lung epithelial cell line (BEAS-2B) and human pulmonary artery endothelial cell line (HPAEC) were treated with lipopolysaccharides (LPS). MTT and flow cytometry were performed to detect cell viability and apoptosis, respectively. Enzyme-linked immunosorbent assay (ELISA) and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to assess the levels of inflammatory factors.Results: Treatment with LPS resulted in the decrease of cell viability in BEAS-2B and HPAEC. CXCL3 was particularly upregulated in LPS-treated BEAS-2B and HPAE cells. Knockdown of CXCL3 enhanced viability and suppressed apoptosis i006E LPS-treated BEAS-2B and HPAE cells. Knockdown of CXCL3 also upregulated TNF-α, I L-1β, and IL-18 in LPS-treated BEAS-2B and HPAE cells. Moreover, knockdown of CXCL3 suppressed the activation of mitogen-activated protein kinases (MAPKs) signaling in LPS-treated BEAS-2B and HPAE cells through downregulation of p-ERK1/2, p-p38, and p-JNK. On the other hand, overexpression of CXCL3 caused completely opposite results in LPS-treated BEAS-2B and HPAE cells.Conclusion: Knockdown of CXCL3 exerted antiapoptotic and anti-inflammatory effects against LPS-treated BEAS-2B and HPAE cells, at least partially, through inactivation of MAPKs signaling, suggesting a potential strategy for the intervention of sepsis-induced acute lung injury (AU)

PLCE1 alleviates lipopolysaccharide-induced acute lung injury by inhibiting PKC and NF-kB signaling pathways

Background: Acute lung injury (ALI) is a clinical syndrome characterized by hyperosmotic ­pulmonary­edema­and­ increased­alveolar­fluid.­Phospholipase­C­ epsilon-1­(PLCE1),­identified­as a member of phospholipase family, and the relationship between PLCE1 and lung injury is n ot clear.Objective: To assess the possible role of Phospholipase C Epsilon 1 (PLCE1) in Acute lung injury (ALI) progression and related mechanisms. Materials and methods:­ The­ effects­of­ LPS­ and­ PLCE1­on­ cell­ viability­and­ apoptosis­were­examined­by­ MTT­ and­ flow­ cytometry.­Also,­ the­ level­ of­ PLCE1­was­ controlled­by­ transfection­of­ its­ plasmid­and­ shRNA.­The­ inflammatory­response­in­ response­to­ PLCE1­overexpression­or­ablation was analyzed by quantitative PCR and ELISA assay. And the involvement of PKC and NF-κB­signal­pathway­were­detected­by­Immunoblot.­Results: In this study, we developed a LPS-induced ALI cell model. We found PLCE1 was upreg-ulated­in­ LPS-induced­pneumonia­cells­ and­ affected­cell­ viability.­Also,­knockdown­of­ PLCE1­reduced LPS-induced apoptosis of pneumonia cells. In addition, depletion of PLCE1 suppressed LPS-induced­secretion­of­ proinflammatory­cytokines­in­ pneumonia­cells.­Mechanically,­we­found­depletion­of­ PLCE1­inhibited­PKC­ and­ NF-κB­signal­pathway,­and­ therefore­alleviated­LPS-induced ALI.Conclusion: We therefore thought PLCE1 co (AU)uld serve as a promising drug for ALI

Application of Multi-element Fingerprints in the Study of Origin Traceability of Anemarrhenae Rhizoma / 中国药师

Objective:To study the origin traceability of anemarrhenae rhizoma from Bozhou and Hebei based on multi-element fingerprints technology , and establish a discrimination model .Methods:The contents of 48 elements were determined by using induc-tively coupled plasma mass spectrometry ( ICP-MS) for 44 samples of anemarrhenae rhizome from Bozhou and Hebei province .Princi-pal component analysis ( PCA) and orthogonal partial least squares discrimination analysis ( OPLS-DA) were applied in the data analy-sis to screen out the significant elements .And then Fisher linear discrimination analysis was used to determine the origin of anemarrhe-nae rhizoma and the discrimination models were developed .Results:Two discrimination models were developed by the discrimination a-nalysis of the whole model method with nine significant elements identified by PCA and OPLS -DA, and 100％correct classification and 95.5％cross validation were achieved by the models .Conclusion: It is a promising approach to classify the geographical origin of anemarrhenae rhizome based on multi-element fingerprints analysis combined with multivariate statistical analysis .The discrimination models are good enough to be applied in the origin traceability of anemarrhenae rhizome.